Primer Design Tool | Momchi's Genetics Tools™

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Paste DNA Sequence (FASTA or plain): Optimal Tm (°C): Max Tm Difference (°C): Minimum Product Length (nt): [Na⁺] (mM): Primer conc. (nM): Number of Primer Pairs (1–20): Optional: Target Region Start (1-based): Optional: Target Region End (1-based): Design Primers

Primer Map

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The Primer Design Tool identifies optimal forward and reverse primers flanking a specified DNA region based on user-defined parameters like melting temperature, GC content, and product length. It provides a detailed results table and a visual map showing primer positions, directions, and the targeted sequence region for easy interpretation. ATENTION: This tool is currently under development; for critical experiments, we strongly recommend verifying the results independently.