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Protein Molecular Weight & pI Calculator | Momchi's Genetics Tools™

Paste an amino-acid sequence (one-letter codes). The tool computes estimated molecular weight, predicted isoelectric point (pI), and plots net charge vs pH.

Results

Molecular weight: — Da

Estimated pI: —

Length: — aa

Charge at pH 7.0: —

Net Charge vs pH

Amino acid counts

Paste a sequence and press Calculate.

Notes & export

This calculator uses standard residue average masses and typical pKa values. It estimates pI by finding the pH where net charge ≈ 0.

This tool analyzes an amino acid sequence to calculate its estimated molecular weight, isoelectric point (pI), and amino acid composition. It also visualizes the protein’s net charge across pH 0–14 with a charge–pH graph, helping identify the pI and charge behavior under different pH conditions.

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